Samples were managed by the sample manager software SampleTrack 2.60.01 (Bruker BioSpin) that allows the printing of unique linear barcodes, necessary for identifying samples, according to their container type.
Fluorescently labelled plasma N-glycans were separated by HILIC on a Waters Acquity ultra-performance liquid chromatography (UPLC) instrument (Milford, MA, USA) with a quaternary solvent manager, sample manager and a FLR fluorescence detector set with excitation and emission wavelengths of 250 and 428 nm, respectively.
Purification of 4-VC was performed on a Waters preparative HPLC system comprising 2767 sample manager module, 2525 binary gradient pump module and 2996 photodiode array detector module.
Customize your throughput and condition requirements with comprehensive system components including the nanoACQUITY® Heating and Trapping Module, the Sample Manager, the Binary Solvent Manager, and the Auxiliary Solvent Manager (optional).
The peptide was purified by semipreparative RP-HPLC [Waters 2487 Dual Absorbance Detector equipped with a Waters 2700 Sample Manager, a Waters 600 Controller, a Waters Fraction Collector, a Symmetry column (C18, 5 μm, 30 × 100 mm2), and Millennium software].
LC-ESI/HRMS was performed on a Waters ACQUITY UPLC system equipped with a binary solvent delivery manager and a sample manager, coupled with a Waters Micromass Q-TOF Premier Mass Spectrometer equipped with an electrospray interface (Waters Corporation, Milford, MA).
The sample set was randomized and held in an 8°C sample manager during the analysis.
The UPLC system included a Sample Manager with Flow-Through Needle (SM-FTN) and a Binary Solvent Manager (BSM).
The UPLC® system (Waters ACQUITY, Milford, MA, USA) consisted of a binary solvent manager, sample manager and UV detector.
All analyses are controlled by a sample manager that tracks samples along the full metabolomics pipeline (labeling, FIA-NMR analysis, spectra preprocessing, and data storage).
