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In pure culture, prey (bacteria) and predator (bacterivorous protozoan) counting was done by sampling in triplicate according to a time series ranging from 0 to 384 h (depending on the grazer species).
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The reproducibility (± 5% for most elements) was checked by digesting one sample in triplicate.
For all plasma assays, standard curve samples were run in duplicate and experimental samples in triplicate.
We tested samples in triplicate in parallel with the housekeeping gene GUSB.
In brief, each plate contained the same reference sample in triplicate.
A total of 500 cells were counted for each sample in triplicate.
The analysis was performed on each sample in triplicate with an ABI 7900-HT (Applied Biosystems, Foster City, CA).
We routinely assayed each sample in triplicate.
We recommend running each sample in triplicate.
We ran each sample in triplicate.
RT-qPCR was performed for each sample in triplicate.
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