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The image gray-scale transformation curve is obtained by sampling image data under the different polarization and positions.
In order to validate this digital method, three 25 × 25 cm areas of one sampling image were printed in color, and on each print, two 5 × 5 cm areas were randomly covered with transparent scale paper.
Although neurite curvature can be quantified using image-analysis software (Kraft et al., 2006; Narro et al., 2007), the method requires immunostaining, random sampling, image acquisition and image processing.
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When left ventricle and right ventricle were used for the image-derived sampling, image-derived blood activity was 81% and 822 % of blood sampling radioactivity, respectively.
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A sample image recorded using holographic interferometry is successfully analysed.
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