When using MeCN as a solvent, the reaction vessel was heated at 80 °C and samples for analytical radio-HPLC were collected during 75 minutes every 15 minutes.
Prolonged treatment (~10 days) with very dilute solutions (~0.005 M) was used to produce the lithium-inserted samples for analytical experiments.
The reaction vessel was then heated at 120 °C and samples for analytical radio-HPLC were collected during 30 minutes every 5 minutes.
This can be performed in hexane/ethyl acetate (10 : 1) to provide a sample for analytical characterisation.
The assumption was that these are independent samples for analytical purposes.
The typical weight of polycrystalline samples for analytical investigations was about 1 g and for single crystal preparation 15 20 g.
A mechanism to converge interests might be to collect water samples for analytical chemistry method development alongside ongoing epidemiological studies, or training analytical chemists in exposure assessment procedures.
Samples for analytical ultracentrifugation (TOG1, TOG2, TOG1-TOG2, and polymerization-competent yeast αβ-tubulin) were dialyzed into RB100 (25 mM Tris pH 7.5, 1 mM MgCl2, 1 mM EGTA, 100 mM NaCl) containing 20 μM GTP.
Samples for analytical ultracentrifugation (TOG1, TOG2, yeast αβ-tubulin, and mutant T238A polymerization-competent yeast αβ-tubulin) were dialyzed into final buffer conditions of RB100 (25 mM Tris pH 7.5, 1 mM MgCl2, 1 mM EGTA, 100 mM NaCl) containing 20 µM GTP.
Normalization will depend on several factors such as sample used for analytical analysis, sample processing, i.e. chemical or metabolic labeling etc.
