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Zebrafish embryos exposed to water and sediment samples from the Argen River from sampling event 1 (a), sampling event 4 (b), and sampling event 5 (c).
Due to financial restrictions, only sediments from sampling event 5 were chemically analyzed.
Due to financial shortcomings, chemical analyses with sediment samples were only conducted with samples of sampling event 5.
Chemical analyses were conducted with fish tissues and sediment samples (analyses of sediments only in sampling event 5).
Juvenile barbels (Barbus barbus; sampling event 3) revealed a good liver condition with only slight alterations of hepatocytes.
A significant variation was found among loaches that were caught at sampling event 4. Here, a higher amount of micronuclei was detected in fish caught downstream.
The trapping effort per sampling event was normally 150 trap-nights per 1-ha plot.
Therefore, subsequent analyses focused on variability of sampling event average concentrations rather than results from individual classrooms.
Two individuals per morphospecies per sampling event were included in the genetic analyses, for a total of 90 specimens.
Thus the effective LOD per sampling event is estimated to be 38 fmol for LE and 494 fmol for AT.
In that season, we collected five replicate samples per station per sampling event (there were three events in 2007) at sites chosen based on previous reconnaissance data.
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