Following the methodology in Hayem-Ghez et al. [13], hyperspectral pseudo-colour images of red and blue watercolour pigment standards from the samples discussed above were rendered by identifying areas of their Vis/VNIR reflectance spectra showing the most significant differences.
High variance between the cmsHA89 samples (discussed above) likely contributes to the lower number of significantly-differing transcripts detected in comparisons involving this accession.
To search for C. reinhardtii or closely related Chlorophyceae species in the water samples discussed above, we mixed algae in the samples with an mCherry/VHH B11 chimera prior to examination by confocal microscopy.
Furthermore, we were not able to find evidence of this gene fusion expressed in the panel of 57 breast cancer samples discussed above, suggesting that it is not recurrent.
In addition to this comparison between the RMe and RMg samples discussed above, we computed all between-strain-within-condition, and between-condition-within-strain Pearson correlations for the BYg, RMg, RMe, and RMg samples [see Additional file 1, Table S4].
AFM analysis was also performed for the three samples discussed above.
This is, however, distinctly smaller than the true density as calculated for the most complete sampling volume discussed above and is therefore an underestimate.
In addition to the general sample preparation points discussed above, each of the individual parts of the eye require a more empirical approach.
Thus, given the relationship between the mPing activity and alteration in cytosine methylation at globally sampled random genomic loci, discussed above, it is pertinent to ask two questions: (1) Are the genomic regions immediately flanking the mPing copies also underwent methylation alteration?
It was not possible to be certain about scanning and sampling the same site, as discussed above, again limiting the comparisons made.
