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Methodologies for collecting data were varied: while some authors applied electronic mailing and waited for spontaneous return, or based their findings on self-reported previous experience, others adopted cross-sectional epidemiological sampling consisting of punctual observations [9].
To assess the extent to which Spiroplasma infection varies within and among species of Drosophila, intensive sampling consisting of 50 281 individuals per species was conducted for natural populations of 19 Drosophila species.
We developed a multivariable, vulnerability-based method for spatial sampling consisting of two descriptive indices: a hazards/stressors exposure index (comprising three proxy variables), and an adaptive capacity/sociodemographic character index (five variables).
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The sample consisted of 48 university students.
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