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We next tested the influence of the number of samples within an expression dataset on the relative performance of the top module detection methods within every category.
This study, however, reveals one major problem when using EcoPlates to evaluate samples within an estuarine or seawater matrix.
For each target gene, the normalized ratio was calculated by setting the median ratio across all samples within an assay at the value of 2. The normalized ratio for each individual was calculated and plotted (Figure 2).
These results argue that while a phylum level diversity of a highly-diverse ecosystem could accurately be described by a single pyrosequencing sampling event, the identity of OTU0.03 could differ greatly between seemingly identical samples within an ecosystem.
At the end of the lyophilization process, samples were either immediately rehydrated or stored, the latter by putting the samples within an aluminum pouch under vacuum and heat-sealing the pouch using vacuum chamber packaging machine Audionvac VMS 163 (Audion Elektro, Weesp, The Netherlands).
We performed whole exome sequencing and copy number analysis for multiple primary and metastatic samples within an individual patient.
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They were used to identify the most promising samples within a combinatorial library.
First, data were temporally smoothed with a median filter (that is, 40 samples within a 200 ms window) to reduce scanner-induced noise59,60.
Given a sufficiently high sampling frequency (i.e., if the number of samples within a window allows all possible motifs to occur more than once), PE can be calculated with high precision, still offering satisfying time resolution.
In addition, we have started a clinical prospective validation study, and we will be able to verify the generalizability of our data using fresh blood samples within a couple of years.
A third-generation medical X-ray CT scanner was used to image the samples within a core holder consisting of an aluminum liner and a carbon fiber overwrap.
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