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To improve ability to sequence low parasitemias, ITS1 PCR products from a further 6 samples with weaker bands, were cloned into CloneJet PCR cloning kit (Fermentas, Vilnius, Lithuania).
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We observed a cluster of 4 samples with weak DC-SIGN binding properties deviating from the other stronger DC-SIGN binding milk samples.
Samples with weak growth on DST were excluded, because of the unreliable readout.
Only three samples with weak immunoreactivity were detected in the normal part of the pancreatic gland.
Samples without FISH signal and samples with weak target signals or those with a strong signal background were the main reasons for most of the non-informative cases.
The staining intensity of the stromal tissue was strong in benign nevi, whereas other lesions displayed moderate to strong staining with few samples with weak intensity.
Data were normalized to levels of 18 S rRNA (primers sequences: F-TTAAGTCCCTGCCCTTTGTACAC; R-CGATCCGAGGACCTCACTAAAC). Average Cq of the samples with weak expression was used as calibrator.
To characterize reactivity of camel serum samples with MERS-CoV in different assay formats, we chose 11 camel serum samples with weak and strong reactivity predetermined by using a simple IFA.
A sample was considered to be HER2 positive when either a strong membrane staining (3+) could be observed by IHC or CISH revealed amplification of HER2 in samples with weak (1+ or 2+) membrane staining at IHC.
Certainly, it is unlikely that the ppp distribution would be uniform if small samples with weak associations were used, and therefore power to detect departure from colocalisation would be reduced.
There was good concordance of the aCGH intensity data in pooled population samples with direct PCR analysis of the LCE3C_LCE3B-del in individual samples, showing a lower frequency of the deletion in the samples with weak hybridization signals with respect to the YRI population (Table 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com