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76 The workflow begins with extraction of genomic DNA from patient samples with targeted panels and exome sequencing requiring enrichment strategies to focus on a subset of genomic targets (usually short DNA fragments [100 500 base pairs] flanked by platform-specific adapters).
For PFOA, we used three spiked samples with targeted means of 80, 240, and 750 ng/mL.
In case of 10-fold diluted samples, the DNA quantity in specimens with fragments of approximately 1500 bp was reduced only gently compared to intact samples (from 39.19 ± 7.79 to 37.07 ± 7.85), but concentrations of samples with targeted fragments of 500 bp and 150 bp were significantly reduced (from 39.19 ± 7.79 to 34.24 ± 7.62 or 27.73 ± 5.68 respectively).
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We then selected the pairs with correlation values in opposite directions between the tumor and normal samples and with targeting relationship predicted by at least one of three databases using MMiRNA-Tar.
The developed HRCA was also efficient for field samples with target cell densities ranging from 10 cells mL−1 to 1000 cells mL−1.
The lack of positive samples with target stool pathogens did not allow a complete determination of the costs and labor associated with routine stool cultures.
GPP c. Laboratories that measure HbA1c should participate in a proficiency-testing program, such as the College of American Pathologists CAPP) HbA1c survey, that uses fresh blood samples with targets set by the NGSP Laboratory Network.
It was implied that A of the blank was always subtracted from A of the corresponding sample with target cells.
It is implied that the absorbance of the blank was always subtracted from the absorbance of the corresponding sample with target cells.
However, an examination of records associated with samples already targeted for GWAS identified several thousand subjects with hypothyroidism.
The residual oil in both samples was targeted with tertiary flooding of 0.3 pore volume polymer slug chased with high salinity seawater for the first sample (BER1) and low salinity ten times diluted seawater for the second (BER3).
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