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EFTEM allowed to map of the elemental composition of the samples with spatial resolution typical of TEM analyses, by selecting electrons with a well defined energy from the transmitted beam.
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OCT provides cross-sectional images of the sample with spatial resolution in the tens of micrometers [ 26], and framing rates of 1000 fps have also been demonstrated [ 6].
SCAT uses allele frequencies from georeferenced samples combined with spatial smoothing methods to generate a geographic map of allele frequency variation.
Samples with similar spatial characteristics and relationships were clustered together at low linkage distances, whilst dissimilar samples are clustered at higher linkage distances (Banoeng-Yakubo et al. 2008).
Interferometric microscopy (IM) can provide complex field information of the biological samples with high spatial and temporal resolution with virtually no photodamage.
Several samples with different spatial features were used.
Samples with high spatial frequency content may not be a suitable subject for this simultaneous measurement.
Coherent anti-Stokes Raman scattering microscopy has emerged in the past decade as a powerful multiphoton microscopy technique for rapid label-free imaging of organic materials and biological samples with submicrometer spatial resolution in three-dimensions and high chemical specificity.
More detailed reconstruction of the wolf population history in Europe demands the analysis of longer sequence data for a more extensive set of ancient samples with comprehensive spatial and temporal distribution.
This approach, based on Fourier ring correlation analysis, can also be used to estimate the resolution obtained in SMLM images, although only samples with definite spatial structures were investigated.
Atomic force microscope (AFM) is unique in that it enables detailed structural analysis of wet protein samples (Müller and Dufrêne, 2008), and recent dramatic improvements in scanning speed now enables real time imaging of conformational changes in protein samples with high-spatial resolution (Ando et al., 2013).
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