Sentence examples for samples with secondary from inspiring English sources

Specifically, we show that staining of these samples with secondary antibody produces no staining, demonstrating that the FOXD1 and HuNu staining shown in Figure 9C is the result of the primary antibody, not due to non-specific secondary antibody binding.

In the 11 primary tumour samples with secondary KIT exon 13 mutation (c.1961 T > C, p.V654A) in the recurrent tumour, minor percentages were seen with the GS Junior (Roche).

A negative control incubated with The full name Phosphate buffered saline moves to line 204 PBS in place of the primary antibody was included for each test sample, with secondary and tertiary antibodies as above.

A multiple myeloma cell line U266 (ATCC, Manassas, VA, USA) were used as positive controls for syndecan-1, and the samples incubated with secondary antibody alone were used as negative controls for HPV16-L1.

Tissue samples stained with secondary antibody alone served as controls.

Control samples stained with secondary antibody alone were analyzed in parallel in each experiment.

As negative control, samples incubated with secondary antibodies only were used.

For each epitope tested by either immunoperoxidase or immunofluorescence, negative controls were represented by samples incubated with secondary antibodies and omitting the primary antibodies from the reaction.

This may explain why RNAzol RT method was unable to extract total RNA from the rhizome and flower samples as RNAzol RT lacked PVPP and β-mercaptoethanol which are crucial for total RNA extraction in samples with rich secondary metabolites [ 25].

NIS relative expression in each sample was calculated as the ratio between the median fluorescent intensity of sample stained with both primary and secondary Abs, and the MFI of sample stained with secondary Ab alone.

As negative control, HIV-1-treated samples were stained with secondary antibody alone, whereas medium-treated samples with both primary and secondary antibodies.