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We present experiments on rail samples with real and machined defects, using EMATs to generate and detect a low-frequency wide-band surface wave.
In the case of samples with real infection, three and two bands were obtained from the first and second reactions, respectively (Additional file 1: Figure S1A-B).
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Fourteen convalescent-phase serum samples with real-time RT-PCR confirmed infection with 2013 subtype H7N9 weRT-PCR confirmed 56–81 years of age from whom samples were collected 12–32 days after onset of symptoms and who were hospinfectionin Shanghai Public Health Clinical Center; control samples with 50 stored serum samples collected in 2013 before the emergence of 2013 subtype H7N9.
We have validated altogether 19 RNA products from the original pooled samples with real-time PCR on Fluidigm GEx array (San Francisco, CA, USA) using Taqman Gene Expression assays for the appropriate RNAs obtained from Applied Biosystems (Carlsbad, CA, USA) (for the full list of validated genes see Additional file 4: Table S4).
Matrix effects, that is, effects that entail different signal intensities and thus an altered slope of the calibration curve when comparing calibration data derived from model samples (calibration standards) with real samples, will impair accuracy and precision of quantitative data and detection sensitivity.
We can study escape at this epitope in detail on serial plasma SIV RNA samples with quantitative real time PCR (qRT-PCR) assays specific for WT virus or the K165R escape mutant (EM) virus [20].
All negative samples with the real time PCR procedure also resulted negative with cultural and conventional PCR methods too.
We analyzed these two samples with a real-time PCR-PNA clamp assay that confirmed the mutation in sample 2768 but not in sample 3000.
The norovirus-positive samples detected with real-time RT-PCR were also analyzed with conventional RT-PCR to allow sequence analysis.
In our study, we investigated oral rinse samples coupled with real-time PCR Taqman assays to detect for HPV types 16 and 18, which is sensitive and specific.
At screening, women underwent face-to-face interviews, blood and urine sample collection with real-time HIV, pregnancy and urinary tract infection (UTI) testing, a pelvic examination with sample collection, and a general physical exam.
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