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We extend previous tests of the PPMS in three important ways: to temperatures as low as 0.4 K; to samples with poor thermal conductivity; to compare uncertainty with accuracy.
Several RNA integrity measures were used in this study to detect samples with poor RNA quality before final analysis.
As can be seen on Table S2, with such a low level of contamination reliable results even from samples with poor DNA preservation can now be obtained.
However, numerous prognostic studies have correlated high uPA protein levels in tumor tissue and blood samples with poor prognosis in many types of cancers [14].
We show in this study that despite reliance on opportunistically collected faecal samples with poor extraction success (46% on average), we can obtain useful population estimates, albeit with moderate 95% confidence intervals.
Three gene lists were used for the enrichment analyses: all 1475 genes reported in the 23 independent GEP studies, the 124 genes reported in at least two GEP studies (independently of consistence in expression change between studies), and the 54 genes reported in at least two GEP studies with consistent direction in gene expression change between samples with poor and good prognosis.
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At this sequence we also observed greater DNA methylation in samples with poorer semen parameters (Figure 1).
Individual samples producing results in <80% of the assays were excluded before statistical analyses in order to eliminate samples with poor-quality DNA and in concordance with Easton et al (2007).
The short-term challenges for NGS in the cancer diagnostic setting are based on the use of focussed sequencing: to reduce costs; to improve technical simplicity and reliability; to enable sequencing of samples with poor-quality or severely limited DNA; and to develop semi-automated, integrated and reliable analysis software that does not require input from an experienced bioinformatician.
One star papers had small samples with poorer quality analysis or were general reviews of the literature.
Figure 3a shows a high-magnification HAADF-STEM image of the GaAs/GaAsBi interface taken from the sample with poor Bi content (S1).
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