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Different experimental tests on lime mortar brickwork were carried out, including Brazilian tests on core drilled masonry samples with one diametral mortar joint and double punch tests on extracted mortar joints.
GBS was recovered from 319 (22.4%) samples with one or both media: 318 on STRB compared to 299 using BA.
All ex-vivo assays were performed on fresh paired samples, with one WT and one 77H sample collected and processed at the same time.
For MfAFLP analysis, 54 primer combinations for E coR I/M sp I (EM) and E coR I/H pa II (EH) with three nucleotide selective primers were firstly screened on 5 random selected samples with one from each population.
The phylogenetic analysis conducted on fragment Li grouped together the majority of extant samples with one ancient sequence obtained from seed S10, while the other two ancient sequences form a well-defined clade with one extant sequence collected on the shores of Tăul dintre Brazi.
Samples with one or fewer freeze-thaw cycle exposure yielded lower 260/280 nm ratio on average (M = 1.61, SD =.25) than samples exposed to 2 or more freeze-thaw cycles (M = 1.79, SD =.07), t (9) = 2.47, p <.05.
Filtering of genes was performed based on whether a gene's read counts had at least two samples with one count per million reads.
Extraction of metabolites followed previously published protocols and included randomisation of samples and extraction in sets of 20 samples with one QC and one extraction blank57,66.
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Samples with one-day exposure time showed high negative values while samples with one-week exposure time appeared closer to the origin of coordinates.
Cd treated samples with one-day of exposure time showed negative values of PC2 which could be related to gills genes expression, while Cd treated samples with one-week of exposure time showed positive PC2 values which could be linked with digestive gland genes.
Although double the number of FIT samples would need to be analysed, the costs of personnel needed for the analysis only increased by a factor of 1.5 because some tasks (eg, patient registration) do not require double the amount of work compared with analysing samples with one-sample FIT screening.
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