Microarray studies utilizing baboon samples with human oligonucleotide arrays have been successfully carried out previously[184], [185].
Hemolytic activity was checked by incubating the tested samples with human red blood cells to determine hemoglobin releasing ability by measuring the absorbance at 540 nm, using 1% Triton X-100 as a positive control.
Cross-species hybridization experiments utilizing rhesus samples with human oligonucleotide microarrays have also been attempted [ 15, 18, 19].
The expression and heterodimeric nature of both molecules was verified through western blot detection of reduced and non-reduced samples with human κ and anti-His specific antibodies.
All 7 samples with human DNA below 940 pg per sample, as measured by the human 18S-PCR, showed mold hyphae.
Finally, to determine the sensitivity and specificity of this assay we conducted experimenter-blinded tests using 35 deidentified, codified samples contained E. coli 0157 H7 and S. typhimurium LT2 (15 of each), as well as 5 samples with human genomic DNA that were negative for bacteria.
