For the difference between paired samples (depletions, blocking experiments, experiments with and without transwells), significance was assessed using the Wilcoxon signed rank test with two-tailed P values.
Zhang et al. (2007) investigated the enhancement of oil recovery by temperature increase in low-permeability (less than 5 mD) chalk outcrop samples with spontaneous imbibition experiments.
Plates were then washed four times with PBS containing 0.1% Tween-20 (Sigma, Stockholm, Sweden) and samples with serial dilutions (experiments were performed in duplicates) in PBS-Tween were added to the plates and incubated overnight.
We initiated our validation of the PLA technique as a tool for measuring protein:protein complex in biological samples with some basic experiments of protocol optimization.
She made kitchens, and bathrooms, and polyglot interiors, arranging and rearranging them, putting in cardboard partitions covered with wallpaper samples, experimenting with light and shadow by shooting at different times of day.
The supernatant was diluted with Na-citrate buffer at pH 4.8 prior to measurements; typically, the dilution factor for samples from experiments with Cellic® CTec2 was 800 to 1,200 and for samples with Novozym 188 was 1,000 to 1,500.
For example, Bonte et al. (2013a) and Javed and Siddique (2016) reported the release of As from rock samples in experiments with elevated temperatures, and showed that the mobilized As had been sorbed to the rock matrix.
To avoid artifactual signals, samples and experiments with poor replicate quality were removed.
This information was then used to correct by subtraction the spectrophotometric readings of effluent samples in experiments with colloids.
Because HBD-2 levels were below the detection limit in most superfusate and supernatant samples, functional experiments with TNF and hormones aimed only to investigate the α-defensin HNP1-3.
