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PCR yielded positive results on samples with as few as 1.08 infected neutrophils/μl (about 0.1%).
However, Svensson's method is found to be very robust and possible to use even in small study samples, with as few as ten to twelve subjects [27].
Validation of the method showed that our real-time PCR based approach could identify N. plumchrus nauplii in field samples with as low as 9% of underestimation, the effect of which is negligibly low on the investigation.
Today the number of 14C atoms in relation to the stable carbon isotopes can be directly measured with an Accelerator Mass Spectrometer (AMS) in samples with as little as 100 μg of carbon, and yet producing precise ages of around ± 40 years for samples being a few 1000 years old.
We tested the robustness of this assay by evaluating FISH on samples with as few as 2 CTCs and many as 159 CTCs (Table 1).
This included those samples with as little as 1 ng of amplifiable DNA per primer pool.
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Even though the tests conducted are preliminary at this stage, sampling with as little as 100 samples per image performs promisingly enough to be further examined.
In fact, the human gut microbiota samples did not contain a single sample with as many unassigned reads as any guinea pig sample.
Emphasis was then placed on having a most representative sampling with as equal as possible distances between the selected cores and equal representation of all tumor foci.
This prescreening allowed us to detect mutations in a heterogeneous tumor sample with as low as 1% mutated cells [ 41], and aberrant migrating band on the TTGE gel can be sequenced directly to define the sequence alteration.
At video rate, pixels in the central field of view are sampled with as few as 12 consecutive laser pulses, each of which corresponds to one of the 10 possible polarization states produced by the EOM.
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