More precise experimental setup and tests with higher enriched samples will be followed to develop a system to apply it to an active measurement for the safeguards of a spent fuel treatment process.
These samples will be followed up throughout their educational career with yearly measurement points.
The nomenclature for the aluminum suspension samples will be as follows: for an aluminum nanoparticle suspension volume fraction of 5% in ethanol, it will be indicated by Eth + 5% Al, or Eth + 5%Al2O33 for alumina.
The sample will be followed up at age 16 to investigate the predictive value of genetic factors and intermediate phenotypes for the development of mental disorders, such as alcohol dependence.
To protect subject confidentiality, blood samples will be anonymized as follows: Last name: All participants will be assigned the last name 'LIBR.' First name: The first name will be a secure alpha cryptographic hash based on LIBR user ID.
The sampling logic will follow the one proposed by Yin [ 60]: Each case must be carefully selected so that it either (a) predicts similar results... or (b) predicts contrasting results but for predictable reasons.
In the larger study to follow, serum samples will be tested for PCBs (congener specific and coplanar), DDE, dioxin, and furans.
Following the same procedure, blood samples will be collected during the follow-up, to assess serum PIIIP concentrations 6 and 12 months after surgery.
Samples will be stored at −80°C, followed by analysis using a commercially available sandwich ELISA according to the manufacturer's instructions (USCN Life Sciences, Wuhan, PRC).
To the dried extract, pentafluorobenzylbromide and 20 μL diisopropylethylamine will be added and incubated at room temperature for 30 min. Following incubation, samples will be further dried under nitrogen to give the F2-isoprostane pentafluorobenzyl ester.
