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The swollen samples were wiped softly to clear away the surface water, and the weight was measured at 30 min interval until equilibrium was reached.
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Initially the sample dimension were estimated and weighed as W i. They were then fully immersed into the solutions, after immersion, the soaked sample were wiped and weighed to obtain swollen weights W s. The samples were dried in an oven at a temperature of 50 °C for 24 h.
This does not disturb their reaction with neuraminidase, but it does allow a large enough accumulation of them to be visible.To find out if a patient has influenza, a sample is wiped from his throat with a swab and added to the kit.
The surface of the sample was wiped with ethanol, and the front surface was coated with graphite emulsion with the temperature of 25 °C.
Lastly, the sample was wiped with filter paper and weighted as W2.
For the wood adherend samples, the surfaces were wiped by acetone.
Splash drops were wiped off.
Then the sample surface was wiped with a piece of ChemWipe paper, in a typical force of 1 N [33], to remove the nonspecifically adsorbed protein on the OTS background, while those specifically bind to substrate surface remained.
The excess fluid was wiped around sample area with absorbent paper.
First, dust wipes cannot be collected as duplicates because once a particular surface has been wiped another sample cannot be retrieved, requiring that a second surface must be wiped.
To reduce nonspecific staining, sections were covered with the sera of rabbit origin (INVITROGEN, Carlsbad, USA) and held on room temperature in wet chamber for 10 min. Sera was discarded and slide was wiped around sample area.
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