Sentence examples for samples were validated using from inspiring English sources
Results from clinical samples were validated using PCR.
Results of selected samples were validated using the Epitect Sequencing Service (Qiagen).
The HBV integration sites identified in these samples were validated using PCR and Sanger resequencing [ 7].
The samples were validated using data distribution and the sources of the variation parameters in the PGS).
CNVs detected in the UCL1 samples were validated using TaqMan® RNase P Copy Number Reference CNRR) Assay, Human (Applied Biosystems).
After each step the samples were validated using an Agilent 2100 Bioanalyzer (Agilent Technologies), and the final concentration was measured using a Qubit 2.0 Fluorometer (Invitrogen).
Differential expression of these genes in either Cr_large or Cr_small samples was validated using quantitative real-time PCR.
The concentration of each sample was validated using the Nanodrop spectrophotometer (NanoDrop Technologies, Wilmington, DE, USA).
Our choice of the 13 genes was based on the fact that they were established from a high throughput technology and a comprehensive study involving both cell lines and clinical white colon samples that were validated using DNA from normal and cancer tissue [10], [11], [33].
The SHH mutation in Sample 1, FLT3 mutation in Sample 3, and DMBT- 1 mutation in Sample 4 were validated using PCR and capillary sequencing.
Microarray results of seven selected genes with differential transcription in CW and OW sampled in autumn were validated using the RT-MLPA method.
