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The eDNA samples were validated by endpoint PCR using the best molecular marker for dreissenid specificity previously selected in Stage A. Gradient PCR with an annealing temperature ranging from 60 to 70 °C and a Touch-Down PCR with decreasing annealing temperature from 70 to 60 °C (−1 °C/cycle in the first ten cycles) were performed in parallel to optimize PCR amplification.
Purity of RNA samples were validated by the absence of MAP locus 251 amplification via PCR.
The predicted aberrations in exome samples were validated by Affymetrix SNP 6.0 data generated for the same samples.
Positive results for all 22 serum samples were validated by microneutralization assay; 15 (68.2%) samples had microneutralization antibody titers of >1 10 against H7N9 virus antigen (Table).
Transcript levels in various tissue samples were validated by qRT-PCR using new total RNA samples prepared from B301 root tissues before and following attempted Striga parasitism as described above.
The cells that were viable were functionally active because they responded to PPD and all these samples were validated by proliferative response to Phytohemagglutinin (data not shown) before they were evaluated using the assays.
FOX gene promoter hypermethylation in patient samples was validated by bisulfite sequencing analysis (BSA).
The integrity of all of the DNA samples was validated by PCR amplification of a 600-bp fragment of the GAPDH gene.
The VHH ELISA for the spiked samples was validated by comparing the results of ELISA with those of an instrumental method LC MS/MS.
The quality of purified DNA from plasma samples was validated by conventional PCR amplification of the humanβ-globin gene using gene-specific primers (βF: 5'-AGGAGTGGTGGCTCATGTCT-3' andβR: 5'-CTCAAGGGATCCTCCCATTT-3').
The very strong induction of dmr6-like in the shoot apical meristem samples was validated by a 41-fold and 202-fold induction in the columnar shoot apical meristem replicates.
