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Samples were validated in lung tissue achieving a validation rate of 65.7% (Supplemental Table S3).
All samples were validated for subsequent analysis with a RIN value of at least 6.0.
All the samples were validated in duplicates.
Results from clinical samples were validated using PCR.
In Ontario, 10% of samples were validated with 100% concordance.
Results of selected samples were validated using the Epitect Sequencing Service (Qiagen).
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The method developed for analyzing the detoxified samples was validated as per ICH guidelines Q2R1.
The online SPE LC/MS/MS method for waters samples was validated in terms of specificity, linearity, limit of detection, matrix recoveries and inter-day precision of the technique.
After optimisation, application on environmental water samples was validated, and specificity towards the targeted virus types was obtained through the qPCR step.
Differential expression of these genes in either Cr_large or Cr_small samples was validated using quantitative real-time PCR.
FOX gene promoter hypermethylation in patient samples was validated by bisulfite sequencing analysis (BSA).
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