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Snap frozen tumour samples were utilised for Western blotting.
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A tissue microarray (TMA) constructed from duplicate 2 mm cores of invasive breast carcinomas from 1290 primary invasive breast cancer samples was utilised for the analysis of CD151 status.
Samples were utilised from two separate trials following informed consent.
Plants for the MDMV experiment were harvested at time points T2 and T3, while T1 and T9 samples were utilised from the SCMV experiment.
To experimentally validate our calls, we verified a subset of potential sites by performing Sanger sequencing of both DNA and RNA from the same samples that were utilised for RNA-Seq.
For both baseline and evaluation surveys, the same methods were utilised for sampling and laboratory diagnosis.
The same aliquots of total RNA samples used in the microarray experiments were utilised for this analysis.
This means that all available samples are utilised both in the training and for estimating the residual error of the model.
Data are reported as mean±standard deviation (s.d).. To determine whether indomethacin had a significant effect on number of tumour nodules per animal and the total tumour volume per animal in combination with a low- or high-LA diet in vivo (Table 3), two-way analysis of variance (ANOVA) for independent samples was utilised.
Paired-samples t-test or Mann-Whitney U tests were utilised for comparisons between groups (depending on the normality of the distributions).
Pathology samples from participants collected for other reasons and suitable for HbA1c testing were utilised for opportunistic diabetes screening.
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