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Samples were treated for 15 min with 0, 25, 83 and 250 μA/cm2 DC.
When the samples were treated for 50 s or even longer, no spores could survive.
The tensile strength of the coatings was about 44 and 24 MPa when the samples were treated for 15 min at 450 V and 480 V respectively.
The samples were treated for 120 min (550 °C and 770 °C) and 360 min (550 °C), with 16 kV high voltage pulses.
Afterward, samples were treated for 5 min with a freshly prepared aqueous solution of APTES (0.5% v/v) (Sigma Aldrich) in order to deposit a cationic layer yielding surface-exposed primary amino groups.
Protein samples were treated for 1 h at 50°C in 2% SDS treating solution.
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The prepared samples are treated for extraction of CeO2 by separating the organo-aqueous phase.
When the stage temperature reached 400 °C, each sample was treated for 5 min. The ionic current was monitored using a current ammeter connected to the process stage.
Total RNA from each sample was treated for potential genomic DNA carryover in a single reaction in accordance with guidelines supplied by Promega (Promega Corporation, Madison, WI, USA).
Each tissue sample was treated for five hours with 200 u/ml of collagenase IV (Eubio, Wothington Biochemicals, #LS004188), and then placed into a culture flask containing FBM (including one FGM BulletKit and 10% FCS) and incubated at 37°C/5% CO2 for about five days until fibroblasts grew out of the tissue.
The samples were treated stepwise for 20 min in mixtures of ethanol and isoamyl acetate with ratios 3 1, 1 1 and 1 3 before soaking in isoamylacetate.
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