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For analysis, samples were thawed.
The plasma samples were thawed at room temperature.
Frozen tissue samples were thawed for 45 min and homogenized.
For NMR analysis, serum samples were thawed on ice.
Serum and urine samples were thawed and vortex mixed.
The frozen tissues from the initial samples were thawed.
Subsequently, samples were thawed to break down mucopolysaccharides that can interfere with pipetting [12].
The snus samples were thawed at room temperature for 1 h prior to extraction.
On the day of analysis, the plasma samples were thawed on ice.
Urine samples were thawed to room temperature and filtered over 0.45 µm syringe filters.
Before drying, olive cake samples were thawed during 24 h under refrigeration conditions at 5 °C.
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