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Samples were submerged in H IP and stored in the dark at 8°C at all times.
In brief, samples were submerged in a digestion buffer with final concentrations of 0.45 M EDTA and 0.25 mg/mL proteinase K (Qiagen, the Netherlands) and rotated at 37 °C until decalcified.
Samples were submerged in Phosphate Buffered Saline (PBS) fluid at 37 °C, in order to mimic in some extent the human body environment.
Pin-on-disk tests were performed under different test atmospheres consisting of dry argon (0% RH) and ambient air (40% RH) and also while the samples were submerged in water (H2O) and ethanol (C2H5OH).
After 4 years, the concrete samples were submerged for 10 days to be fully saturated.
Subsequently, the samples were submerged in a HF/H2O2 aqueous solution for MaCE.
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Subsequently, the sample is submerged in a solution of Cu+ cations in methanol (4 mL, [Cu+] = 75 mg/mL) for 5 min at room temperature to trigger the CdSe→Cu2Se CE transformation.
When the sample is submerged, ΔM is equal to the weight of water that is displaced.
Specimen collection was performed immediately after the CT examination; the sample was submerged in 10% buffered formalin and delivered to the laboratory for histological analysis.
After plasma treatment, channels were removed and the sample was submerged in a suspension of human umbilical vein endothelial cells (HUVECs) for 24 h to allow homogeneous and geometry-independent cell seeding.
After washing it 3 times for 5 minutes, the sample was submerged by diluted gout secondary antibodies (2 mg/mL) 1 : 1000 with the same PBS solution in dark room at room temperature for one hour.
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