Sentence examples for samples were stopped by from inspiring English sources

Depending on the analytical method, the samples were stopped by heat or by adding conc.

Reaction samples were stopped by mixing with loading buffer (95% formamide, 35 mM EDTA [pH 8], 0.1% bromophenol blue, 0.1% xylene cyanol).

RFB assay samples were stopped by spotting 20 μl on to P-81 phosphocellulose filters pre-wetted with 0.75% phosphoric acid.

Also, the samples were stopped by the addition of 2X Laemmli buffer, separated on 10% polyacrylamide gels and probed by immunoblotting with anti-PAR antibodies (Trevigen) and reprobed with anti-PARP-1 (Cell Signaling) and anti-NEIL1 (Calbiochem) antibodies.

Gel assay samples were stopped by the addition of reducing/denaturing loading buffer, then boiled for 10 min, quickly cooled, and loaded into 30 μl wells on a 10% precast Tris-glycine gel (Bio-Rad Laboratories).

At definite intervals, samples were taken from the reaction mixture, and the reaction in the selected samples was stopped by boiling for 5 min.

The colour-developing reaction in serum samples was stopped by adding 50  μL of 2 M sulphuric acid and the OD (450 nm) was measured in a plate reader.

Samples (50 µL) were stopped by adding 5 µL of 20% formic acid and diluted with 50 µL water.

At different points of time, samples were taken and reactions were stopped by centrifugation (24,725 × g, 20 min, 4°C) of resting cells and immobilized peptidases, or by heat treatment (1,000 rpm, 90°C, 10 min) followed by centrifugation (24,725 × g, 20 min, 4°C) of crude extract and enzymes.

Reactions were stopped by adding sample buffer and samples were analysed by SDS PAGE and Instant Blue staining.

Samples were incubated at 37°C and reactions were stopped by boiling in SDS sample buffer prior to western blot analysis.