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For this purpose tensile tests of the respective samples were stopped at forces far below the yield point and subsequently 3D reconstructions of the fracture regions were performed.
Depending on the analytical method, the samples were stopped by heat or by adding conc.
Reaction samples were stopped by mixing with loading buffer (95% formamide, 35 mM EDTA [pH 8], 0.1% bromophenol blue, 0.1% xylene cyanol).
RFB assay samples were stopped by spotting 20 μl on to P-81 phosphocellulose filters pre-wetted with 0.75% phosphoric acid.
Also, the samples were stopped by the addition of 2X Laemmli buffer, separated on 10% polyacrylamide gels and probed by immunoblotting with anti-PAR antibodies (Trevigen) and reprobed with anti-PARP-1 (Cell Signaling) and anti-NEIL1 (Calbiochem) antibodies.
Gel assay samples were stopped by the addition of reducing/denaturing loading buffer, then boiled for 10 min, quickly cooled, and loaded into 30 μl wells on a 10% precast Tris-glycine gel (Bio-Rad Laboratories).
Similar(53)
At definite intervals, samples were taken from the reaction mixture, and the reaction in the selected samples was stopped by boiling for 5 min.
The colour-developing reaction in serum samples was stopped by adding 50 μL of 2 M sulphuric acid and the OD (450 nm) was measured in a plate reader.
In addition, it can be (and usually is) called after the sampler was stopped.
A stopping criterion is proposed such that the estimates satisfy a prescribed precision when the sampling is stopped.
Once the computation is done and the sampler is stopped, the accumulated data is analyzed (by analyze-samples) and the resulting profile value is sent to the renderer function.
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