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Samples were stocked at −80°C prior to the analysis.
Samples were stocked at −80 °C until Bio-Plex cytokine assay.
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Following baseline sampling, rainbow trout (Oncorhynchus mykiss) were stocked into the CAGE-FISH and the CAGE-FISH-EXCL treatments at an initial stocking density of 2.4 kg m−3.
The new store, a cross between an art gallery and a fashion sample room, is stocked to its 13-foot ceiling with wide rolls of fabrics, lace and rickrack trims, pearl buttons and finished sample garments.
Actually, some of their parents (hence grandparents of the fry that we sampled) could had been stocked in the Malbaie River in the 1990s.
Records indicate that one of our sampling sites (SKT) was stocked with brown and rainbow trout in 1985, but surveys after 2000 indicate that these introduced species are no longer present in this lake.
At first, our study was retrospective, and patients whose blood samples were not stocked were excluded.
Among lakes sampled between 1974 and 1983, three were never stocked and one was stocked with juveniles produced with its own broodstock after 1962.
Stock samples were stored at -20 C. For each assay the samples were diluted in the assay buffer supplied with each individual kit.
Earth concrete samples were cast then stocked at normal room temperature and humidity levels.
Multiple samples were collected and stocked at the DNA bank at Southern Cross University, New South wales, Australia under approval of EcoAccess published by the Queensland state government.
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