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Liver samples were separately collected from each group by the end of each month.
Accurately weighed, 0.5 g of both the mushroom and substrate samples were separately digested, each for 2 min.
Over the test period, the samples were separately conditioned at 15 °C, 20 °C, 30 °C and 35 °C.
To ascertain the binder creep phenomenon, samples were separately conditioned at 15 °C, 20 °C, 30 °C and 35 °C.
For heavy metal analyses, samples were separately collected in pre-washed polypropylene containers of 100 ml capacity and acidified onsite to avoid precipitation of metals.
The samples were separately mixed with accurately weighed antioxidants to form different concentrations of the antioxidant solution in parts per million (ppm).
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Then, each of the samples is separately located in pre-simulated environment.
Prior to electrophoresis two protein samples are separately labelled with a heavy or a light version of the CCAT reagent via reduced cysteines in the proteins.
The order of processing and analyzing samples was separately randomized within each block.
Total RNA from 140 μl clinical samples was separately extracted using QIAamp Viral RNA Mini kit (Qiagen, Valencia, CA, USA).
The samples with antioxidants and control sample were separately subjected to oxidative stability test in PetroOxy equipment.
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