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The students' instructor proctored all of the task administrations and all speech samples were scored by trained teacher-raters at the IEP.
Two replicate F2 samples were scored.
Samples were scored twice to ensure accuracy.
Samples were scored from duplicate tissue culture wells.
The staining pattern of all biopsy samples were scored as positive or negative as described (Methods).
Samples were scored as positive if CoV RNA was detected on two PCR runs.
This was supported when independent round two, stage I samples were scored by the model with an 85.7% accuracy.
The 34 samples were scored for presence (1) or absence (0) of EcoRI/MspI and EcoRI/HpaII fragments.
These samples were scored negative if no amplicon was acquired through conventional PCR for HAdV-3, -4, -7, -11, -14, or –21.
It was also noted that in a small number of cases, there was sporadic cytoplasmic staining and these samples were scored as negative when nuclear staining was absent.
Samples were scored positive when more than 10% of the cells reacted with the anti-BMI-1 antibody and presented cytoplasm staining.
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