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Mixed samples were reduced to approximately 40 µl.
Samples were reduced with tris- 2-carboxyethyl) phosphine (5 mM) and alkylatris- 2-carboxyethylanetris- 2-carboxyethylM).
Protein samples were reduced by adding tris 2- carboxyethyl phosphine to a final concentration of 5 mM, followed by incubation at room temperature for 30 min.
Before some of the subsequent characterizations, the samples were reduced in pure hydrogen at 550 °C, converting NiO into Ni, which could better simulate the real operating conditions.
The samples were reduced with H2 without or after calcination in air or helium.
Negative matrix effects due to the complexity of the studied samples were reduced by dilution with ultrapure water.
In short, protein samples were reduced and alkylated with iodoacetamide and trypsin digested, subjected to nano-liquid chromatography on an Ultimate 3000 system (Dionex) and subsequent MS/MS analysis on an Impact QTOF instrument (Bruker Maxis).
Both Pd technologies stored more NO when the samples were reduced, indicating that metallic Pd is effective for storing NO.
With 15 W UV-C radiation, algal concentration of three samples were reduced to 75.3%, 51.5%, and 70.0% of the initial level within an hour, respectively.
Samples were reduced with hydrogen at 500 °C and then nitrided at 300 °C in gaseous ammonia-hydrogen mixtures of different nitriding potentials.
Protein samples were reduced, alkylated and digested with trypsin.
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