Exact(3)
After washing in phosphate buffer solution with Tween20 (PBST) for 30 min, the TNF-α samples were reacted for 30 min by indirect immunostaining using anti-goat antibody conjugated with horseradish peroxidase (P0449, diluted 1 30, DAKO, Tokyo, Japan).
Samples were reacted for 1 h at 37°C and processed as described above.
Samples were reacted for 5 min with 3%H2O22 in PBS to deplete endogenous peroxidase activity and then washed with PBS.
Similar(57)
For the light-driven transcription-and-translation reaction, the sample was reacted for 13 h and subjected to column filtration (RNase-Free Micro Bio-Geln Columns with Bio-Gel P-30).
The diluted serum samples were reacted to the peptide-coated microtiter wells for l h at 37°C.
Powdered samples were reacted with >100 % phosphoric acid at 80 °C for >12 h using a Thermoquest GasBench II.
After blocking with 1% foetal calf serum, the samples were reacted with a 1∶10,000 dilution of group 19 typing sera (Statens Seruminstitut, Copenhagen, Denmark) for 4 h.
Samples were reacted by individual acid addition (99%H3PO4at73 73 °C).
The samples were reacted with anti-P.
Bile samples were reacted to H. pylori and H. hepaticus by Western blotting.
Ferritin was injected into the rat vasculature and allowed to circulate for 5 min. Samples obtained from tibiae were reacted for different times with Perl's reagent and then were either paraffin-embedded or sectioned with a cryostat.
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