Sentence examples for samples were quantitated using from inspiring English sources
Total RNA samples were quantitated using the fluorescent Ribogreen RNA quantitation reagent kit (Molecular probes) and RNA was stored at −70°C until use for RT PCR.
All samples were quantitated using the comparative CT method for relative quantitation of gene expression, normalized to β-actin and 18S [ 24, 25].
Baseline aflatoxin levels and 10× concentrated mushroom samples were quantitated using the Neogen® Reveal® Q+ test kit and AccuScan Pro Reader (Neogen Corp., Lansing, MI) per manufacturer instructions.
Differences in mRNA levels between samples were quantitated using the standard curve method.
Reactions were performed in triplicates using iQ Supermix Bio-Radd, Cat. No. 170-8862), and the samples were quantitated using an iCycler iQ Multicolor Real-Time PCR Detection System (Bio-Rad).
All DNA samples were quantitated using a Nanovue spectrophotometer (GE LifeSciences, Piscataway, NJ, USA).
Following RNA isolation each of the samples was quantitated using a Nanodrop spectrophotometer.
The fluorescence of the samples was quantitated using a standard curve of FITC-dextran (0.4 25 μg ml−1).
The NPTII activity in leaf samples was quantitated using a commercial Patho Screen NPTII ELISA kit (Agdia Inc., Indiana, USA).
The proteins from each sample are quantitated using Bradford or BCA reagents and compared with bovine serum albumin (BSA) standards for accurate protein estimation.
Serum sample Survivin concentrations were quantitated using a commercially available human Survivin Immunoassay kit (R&D systems, Minneapolis, MN) according to the manufacturer's instructions.
