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Total RNA samples were quantitated using the fluorescent Ribogreen RNA quantitation reagent kit (Molecular probes) and RNA was stored at −70°C until use for RT PCR.
All samples were quantitated using the comparative CT method for relative quantitation of gene expression, normalized to β-actin and 18S [ 24, 25].
All samples were quantitated by the comparative cycle threshold C t) method for relative quantitation of gene expression, normalized to either GAPDH or β-actin [ 35].
Baseline aflatoxin levels and 10× concentrated mushroom samples were quantitated using the Neogen® Reveal® Q+ test kit and AccuScan Pro Reader (Neogen Corp., Lansing, MI) per manufacturer instructions.
The lactose levels in 20 breast milk samples were quantitated by the plasmonic MS and compared to the conventional biochemical method (Fig. 3c, Table S3).
ChIP samples were quantitated by real-time PCR.
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The resultant absorbance of samples was quantitated at 450 nm using multimode plate reader (infinite M200, TECAN, Austria), and background was subtracted.
AFB1 from other samples was quantitated via thin layer chromatography (TLC) by fluorometric measurement with a CAMAG TLC Scanner 3 densitometer (CAMAG Scientific, Inc., Wilmington, NC) (Stoloff and Scott 1984; Pons et al. 1966).
Following RNA isolation each of the samples was quantitated using a Nanodrop spectrophotometer.
In addition, a subset of DNA recovered from serum samples was quantitated by the DNA dip-stick test (Invitrogen).
The fluorescence of the samples was quantitated using a standard curve of FITC-dextran (0.4 25 μg ml−1).
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