Samples were quantified using a Picogreen assay on a TBS-380 Mini-Fluorometer.
Vitamin D status was assessed by measuring the storage form 25OHD.34 Samples were quantified using isotope dilution liquid chromatography-tandem mass spectrometry.
Samples were quantified using an Agilent Technologies 1100 Series HPLC equipped with a Zorbax® C18 Stable Bond column.
After purification, the samples were quantified using a Nano Drop spectrophotometer (Nano Drop Technologies, Wilmington, DE, USA).
RNA samples were quantified using spectrophotometry.
Bands from tumor samples were quantified using NIH1.6 Image software.
Protein samples were quantified using the BioRad Protein assay.
Total protein levels of the samples were quantified using Bradford analysis prior to gel loading.
Purified DNA samples were quantified using the Nanodrop 1000 spectrophotometer (Nanodrop Technology®, Cambridge, UK).
RNA samples were quantified using Multiskan Spectrum (Thermo, Electron Corporation) prior to use.
Viral genome of extracted DNA and some cDNA samples were quantified using limiting dilution PCR [26], [27].
