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Pooled samples were purified using calibrated Ampure XP beads.
The adaptor-ligated RNA samples were purified using 2.4-fold excess AMPure XP beads (Agencourt).
Thereafter, all protein samples were purified using a Nanosep® 100 kDa molecular weight cut-off filter (Pall Corporation) to remove any pre-existing aggregates.
The RNA samples were purified using the SV Total RNA Isolation System (Promega, Fitchburg, WI, USA).
The duplicate PCR products were pooled, the DNA concentrations were normalized and the samples were purified using the Agencourt AMPure system (Beckman Coulter).
Samples were purified using a QIAquick gel extraction kit.
All samples were purified using ZipTip (Millipore) prior to MS analysis.
The extracted samples were purified using 0.20-µm, PTFE, HPLC syringe cartridge filters (DISMIC-13JP, ADVANTEC).
All samples were purified using ZipTip® (Millipore) prior to mass spectrometric analysis.
The concentrated samples were purified using the sucrose-density gradient zonal centrifugation (CP-80, Hitachi, Japan).
Samples were purified using Ampure beads (Agencourt) and separated on a native 6% polyacrylamide gel.
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