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The samples were propagated in specific pathogen free embryonated chicken eggs, and the allantoic fluid was subjected to qRT-PCR.
When original clinical samples were propagated in shell vials, inclusions remained small and the rate of growth was similar as for the plaque assay.
After several years of storage the samples were propagated in cycloheximide treated McCoy cells (without antibiotics) in shell vials and then inoculated on to 25 cm tissue culture flasks.
Prior to phenotypic NAI sensitivity testing, fecal samples were propagated in SPF ECE (Valo, Germany), as described above, to obtain a sufficient viral titer for the assay, followed by NA sequencing (as described above) to verify the genotype.
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At the cloud level communication is slower and only periodic samples are propagated.
In (a), samples are propagated following a truncated Gaussian distribution centered at with covariance matrix bounded between and (green zone).
These samples are propagated across iterations in time in a way that capitalises on an application-specific non-linear, non-Gaussian state-space model.
Therefore, the question is selected and subsequent split is achieved differently as shown in Figure 3. First, only the true samples are required to answer the question and the false samples are propagated to both child nodes.
Then, these new samples are propagated and some discrete noise is added to their position meaning that their new positions are also constrained to occupy a discrete 3D coordinate (see an example in Figure 6).
Where N T y and N a l l y are the numbers of the true and all samples at child yes, N T n and are the numbers of the true and all samples at child no, respectively, as shown in Figure 2. N a l l y and N a l l n samples are propagated to further nodes from the child node yes and the child node no, respectively.
Each sample is propagated from the set s ' t − 1 according to s t n = g s t ' n + E (6).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com