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Samples were processed with the Illumina Infinium HumanMethylation450 BeadChip (Illumina, Inc .. Data pre-processing (signal correction and data normalization) and QC were performed using standard criteria described elsewhere [ 33].
All samples were processed with Halosperm for the SDF analysis.
All samples were processed with four biological replicates.
To control for secondary immunoglobulins, samples were processed with omission of the primary antibody.
To control for secondary immunoglobulins, the samples were processed with omission of the primary antibody.
Following exposure, the samples were harvested and the samples were processed with the CLPA and the gene expression was analyzed on the CE instrument.
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The samples corresponding to the cyclic prefix are discarded, and the samples are processed with a fast Fourier transform (FFT).
Part of the samples was processed with red blood cell lysis buffer and part without to further investigate the effect of using this buffer on the concentrations determined.
Overall, it does not appear that a high-percentage of quality control (QC) samples was processed with study samples.
After collection, all samples are processed with the QIAGEN RNeasy Kit to extract the RNA (mechanical disruption protocol with on-column DNase digestion).
A portion each of the stool samples was processed with a direct microscopic technique to detect cysts, trophozoites, eggs and larva of intestinal parasites immediately.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com