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The moose liver samples were pooled by the sampling area, sex and age.
Quadruplicate samples were pooled after extraction.
From this stock, eight samples were pooled in equal volumes.
Feces samples were pooled by cow.
Equimolar amounts of DNA samples were pooled (100 ng/ul per sample).
Field replicates of gas samples were pooled per plot for statistical analysis.
DNA libraries prepared from eight samples were pooled for each MiSeq run.
At least 4 electroporation samples were pooled to ensure full coverage of the bar codes.
Samples were pooled, acidified with 10% TFA to a pH ∼2 and subjected to SPE.
Multiple samples were pooled together in equal proportions based on their molecular weight and DNA concentrations.
Samples were pooled and sequenced using either 76 or 101 bp paired-end reads.
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