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The samples were next desalted using Sep-Pak C18 Vac cartridges (Waters, Milford, MA, USA).
The two sets of samples were next subjected to the uniaxial compression test.
The samples were next conditioned to different levels of relative humidity (0, 64, 89%) before being sealed in d.s.c.s.c
The samples were next treated in Ar atmosphere for 1 10 h at up to 1400 K under HP ≤ 1.4 GPa and investigated by mass spectrometry, photoluminescence, X-ray, electrical and microhardness methods.
These two nanolubricating oil samples were next tested under an increased load of 80 N and the same rotation speed of 350 rpm to examine their tribological performance under a higher load condition, and the corresponding COFs and wear scar images are shown in Fig. 8.
The various HS granulation samples were next compared to the NB cultured from DMEM inoculated with 10% HS (Table 2).
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Each of the samples was next mixed with streptavidin-coated magnetic beads 2 mL (Promega, Fitchberg, WI), followed by incubation for 10 min at room temperature.
The samples are next placed in 10% neutral-buffered formalin (Shandon Formal-Fixx, Thermo Scientific, Waltham, MA) for a maximum of 90 min. This step enhances the red/pale contrast between viable AAR and AON (see Fig. 2, top).
An overview of the demographics and information of the participant sample is next.
The permethylated sample was next extracted into 1 mL of chloroform and washed with 4 × 1 mL of water.
The sample was next extracted twice with cold (-20°C) 100% acetone by vortexing and centrifuging, as before.
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