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Samples were manually introduced to the instrument using a syringe.
For each condition, duplicate samples were manually counted in each of two independent experiments.
Low coverage samples (<0.05×) and low tumor fraction samples were manually reviewed for tumor fraction estimation.
Tissue samples were manually disrupted using a Tissue Lyser II (Qiagen) mixing 2 × 10 s at 20 s−1.
Additionally, gait samples were manually classified into seven carrying status (CS) labels.
For the electroluminescence (EL) measurements, the samples were manually bonded using silver paint.
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To be able to assess the effect of the automatic size selection, one of the automatically prepared cancer cell line samples was manually size selected by agarose gel separation as specified in the standard protocol.
Any foreign material/debris from the soil samples was manually removed during the sample collection.
Approximately 20 μL of the samples was manually injected each time.
We found 5 sample tracking errors in 229 samples; sample tracking errors are expected when large numbers of samples are manually handled [ 21, 22].
All blood samples are manually processed and aliquoted immediately after collection by a trained technician or research assistant according to standard operational procedures, and then temporarily stored at the local research location.
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