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All genomic DNA samples were isolated from anticoagulated peripheral blood using a Puregene DNA isolation kit (Gentra Systems) in the same laboratory as previously described (32).
All planktonic and biofilm samples were isolated from the same experimental system and used for both RNA and protein isolation.
Bacterial samples were isolated from the digesta collected from the omasum.
Plasma samples were isolated from whole blood EDTA tubes after two-step centrifugation: 300×g for 10 min and 3000×g for 10 min.
Over a period of 20 years, a total of 207 Mannheimia haemolytica samples were isolated from calves affected with pneumonic pasteurellosis and serotyped by the indirect haemagglutination test.
Freshly prepared RPE/choroid and RPE samples were isolated from human donor eyes.
Bacterial samples were isolated from the wastewater and soil found at the Muteh gold mine (Isfahan-Iran).
The DNAs of 49 samples were isolated from 4 mL of frozen blood samples using the Gentra Puragene Blood Kit (Qiagen, Hilden, Germany) according to the manufacturer's protocol.
Eight fungal species and ten bacterial samples were isolated from drinking water supply PVC pipelines in Tirupati, Chittoor district, AP, India.
Briefly, poly-A mRNA samples were isolated from the total RNA using oligo (dT) attached magnetic beads and then interrupted into 200 500 bp fragments.
Samples were isolated from patients and characterized by pathology.
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