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The samples were inoculated with 8 common indoor moulds.
The samples were inoculated on modified Mitis-Salivarius-Bactracin (MSB) agar plates.
Sterile "foie gras" samples were inoculated with 14 LAB strains from the collection.
Duplicate samples were inoculated with a mould cocktail consisting of equal proportions of Aspergillus niger and Penicillium spinulosum spores (5·0×104spores/ml).
10, 1 and 0.1 ml of appropriately diluted samples were inoculated into respective dilution tubes containing inverted Durham's tubes.
The samples were inoculated on MacConkey Agar (Difco laboratories, USA) and incubated aerobically at 37°c for 24 h.
Approximately 0.1 ml of filtered and non-filtered aliquots from each samples were inoculated in wells and incubated for 7 days at 40 °C under aerobic conditions.
When the samples were inoculated with a low inoculum of 10 CFU/ml, all samples (except for peach puree) showed detectable Alicyclobacillus spp. within 24 hours.
The samples were inoculated with 2 ml of spore suspension prepared from the subculture and fermented at 24 °C for 12 days.
Initially, the collected samples were inoculated on yeast extract peptone dextrose agar plates and the colonies so obtained were culturally and morphologically characterized.
For the determination of EC20 and EC50 the samples were inoculated with Vibrio fischeri (LCK 482, Hach Lange GmbH, Düsseldorf, Germany) and incubated for 30 min.
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