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Many S. chartarum samples were initially negative after PCR amplification.
Samples were initially ground using a mixer mill.
Core samples were initially saturated with reservoir oil of 3.81 cp viscosity.
The samples were initially cut with a Leco MSX255M saw equipment.
The samples were initially subjected to one cycle load up to 1% strain to determine the creep load.
The samples were initially screened for background Alicyclobacillus contamination per the International Federation of Fruit Juice Producers method (IFU, 2007).
Powder samples were initially characterized using a Hitachi TM1000 tabletop scanning electron microscope (Chiyoda-ku, Japan) working on backscattered mode.
The fault rock samples were initially collected in large blocks with each edge more than 15 cm.
Briefly, samples were initially extracted by 1 mL of 14% boron trifluoride with heptadecanoate as standard in toluene.
Hair samples were initially decontaminated by rinsing twice with 2 mL dichloromethane for two minutes at room temperature.
For identification, collected samples were initially dried and then sample slides (using thin films) were developed for each mosquito species.
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