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Tissue samples were immersed in 10% buffered formalin and prion infectivity was inactivated by immersion into 98% formic acid for one hour.
Samples were immersed in cold absolute methanol for 10 min and treated with 1% Triton X-100 for 30 min.
Samples were immersed in distilled water for various period which included 7, 14 and 21 days.
The stacked samples were immersed in liquid nitrogen in an open cryostat.
Ten samples were immersed in orange juice as a positive control group and another ten samples in water as a negative control group.
Shape memory tests were carried out while the samples were immersed in varying compositions of water/ethanol mixtures.
Samples were immersed in a 10 wt% sodium chloride solution for 91 days.
In the second step, these samples were immersed in hydrofluoric acid to obtain MgF2 coating.
The samples were immersed in water during the static fatigue tests.
The samples were immersed in octane at the desired temperature after which foaming started immediately.
The samples were immersed into the KOH solution.
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