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The samples were immediately stored at −80 °C.
The samples were immediately freeze-clamped in liquid nitrogen with pre-cooled aluminium tongs.
All collected colonic samples were immediately frozen in liquid nitrogen and stored at −80 °C.
All the samples were immediately suspended in 1 ml Trizol reagent (Gibco) for RNA extraction.
After incubation, the samples were immediately analyzed by FACSCalibur Flow Cytometer (BD Biosciences, San Jose, CA).
The saliva samples were immediately stored at −20 °C until the batch assay.
The samples were immediately frozen (−70°C).
Samples were immediately filtered and preserved as needed.
Blood samples were immediately centrifuged and stored in the refrigerator.
All samples were immediately stored in polyethylene containers until diagnostic.
After cleaning, the samples were immediately loaded into a chamber.
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