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Stool samples were frozen and shipped to labs for microscopic evaluation or antibody tests.
All the samples were frozen at −30°C until use.
All samples were frozen at −70 °C for later analysis.
Tissue samples were frozen for PGE2, SOD, and MDA determination.
Plasma samples were frozen at −70°C.
Samples were frozen immediately until use.
The samples were frozen and dried as described above.
Subsequently, samples were frozen in OCT (Sakura, Torrance, CA, U.S.A).
After brief vortexing, the samples were frozen in liquid nitrogen.
Tissue samples were frozen in −80°C until assay.
The samples were frozen and thawed only once.
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