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Cultured samples were fixed in 4% paraformaldehyde, frozen and sectioned prior to histological evaluation.
For flow-cytometric analysis of mouse lung and BALF, samples were fixed using 4%PFA.
Skin samples were fixed in 10% formalin, embedded in paraffin, and cut into 7-um sections.
Samples were fixed with 3% paraformaldehyde plus 0.5% glutaraldehyde as described above.
Samples were fixed with 4% paraformaldehyde at 37 °C for 20 min.
Tissue samples were fixed in 4% formalin and routinely processed into liquid paraffin.
For paraffin embedding, tissue samples were fixed with 4% formaldehyde in PBS.
All water samples were fixed with HgCl2 pending nitrate + nitrite (NO3 + NO2) analysis.
The samples were fixed in 4% formaldehyde.
The samples were fixed inside an inert mounting fixture (polyamide).
Samples were fixed in 4% paraformaldehyde overnight.
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